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Titer is the amount of


A) antigen in serum.
B) antibody in serum.
C) WBC in serum.
D) complement in serum.
E) memory cells in serum.

F) A) and D)
G) B) and C)

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Which test is used to confirm scarlet fever?


A) ELISA
B) Western blot
C) Widal test
D) complement fixation
E) antistreptolysin O

F) A) and B)
G) A) and C)

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The primary advantage of genotypic methods of identification is


A) they are easier than any other method.
B) they are less expensive than other methods.
C) they are widely available.
D) culturing of the organism is not required.
E) All of the choices are correct.

F) C) and D)
G) A) and C)

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In precipitation tests,the antigen


A) is a soluble molecule.
B) is an insoluble molecule.
C) is a whole cell.
D) antibody complex settles to the bottom of the tube.
E) None of the choices is correct.

F) A) and C)
G) A) and E)

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A positive indirect ELISA result requires


A) one known antibody,one unknown antibody,and an unknown antigen.
B) a known antigen,an unknown antibody,and a known antibody
C) two known antibodies and one known antigen.
D) two known antibodies and one unknown antigen.
E) All of the choices are correct.

F) None of the above
G) B) and D)

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Please read the clinical scenario, and then answer the question that follow to become familiar with the traditional NCLEX question format. Halley is a 62-year-old female, previously healthy with no significant medical history, and no known allergies. She is admitted to your medical-surgical floor for an infectious disease work-up. Halley has been experiencing generalized pain and nausea for the past 3 weeks, and recently developed a fever. The doctors at the urgent care center, and then the emergency department, were perplexed by her presentation. You have been ordered to perform several tests: complete blood count (CBC) , C-reactive protein (CRP) , eosinophil sedimentation rate (ESR) , blood culture, urine culture, and a lumbar puncture with cerebral spinal fluid (CSF) culture and cell count. You are also collecting a sputum culture, as well as swabbing her nose with a culturette, and performing a nasal wash. -Halley's CRP came back markedly elevated.What finding does this point to?


A) An elevated CRP is a highly sensitive urinary tract infection marker.
B) An elevated CRP points to inflammation from any of a variety of sources.
C) An elevated CRP shows elevated levels of vitamin C in the blood.
D) An elevated CRP points to a bacterial infection and nothing else.

E) B) and C)
F) None of the above

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The property of a test to detect only a certain antibody or antigen,and not to react with any others is


A) cross reaction.
B) agglutination.
C) precipitation.
D) specificity.
E) sensitivity.

F) A) and E)
G) B) and C)

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Specimen collection


A) is always done by a medical professional.
B) must be done under sterile conditions.
C) must utilize aseptic techniques.
D) does not require special handling.
E) All of the choices are correct.

F) All of the above
G) B) and E)

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Serotyping of pneumococcus based on capsular polysaccharide is performed in


A) Ouchterlony double diffusion.
B) Western blot.
C) immunelectrophoresis.
D) radioimmunoassay (RIA) .
E) the Quellung test.

F) B) and D)
G) B) and C)

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Which test is used to confirm rheumatic fever?


A) ELISA
B) Western blot
C) Widal test
D) complement fixation
E) antistreptolysin O

F) B) and C)
G) A) and E)

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Which test is the confirming test for people who initially tested antibody-positive in the screening ELISA test for HIV?


A) Ouchterlony double diffusion
B) Western blot
C) immunelectrophoresis
D) radioimmunoassay (RIA)
E) Quellung

F) B) and E)
G) All of the above

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Which of the following is not a genotypic method of identification?


A) rRNA sequencing
B) PCR
C) biosensor
D) direct antigen testing
E) DNA analysis with probes

F) D) and E)
G) A) and D)

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Which of the following is involved in the in vitro diagnostic testing of serum?


A) mycology
B) hematology
C) serology
D) histology
E) virology

F) A) and B)
G) A) and C)

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Which of the following is not a phenotypic method of identification?


A) morphology
B) Gram stain reaction
C) acid-fast reaction
D) antibody response
E) endospores

F) D) and E)
G) A) and E)

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Horseradish peroxidase and alkaline phosphatase are enzymes used in _____ tests.


A) Western blot
B) ELISA
C) direct fluorescent antibody
D) indirect fluorescent antibody
E) None of the choices is correct.

F) B) and E)
G) All of the above

Correct Answer

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Please read the clinical scenario, and then answer the question that follow to become familiar with the traditional NCLEX question format. Halley is a 62-year-old female, previously healthy with no significant medical history, and no known allergies. She is admitted to your medical-surgical floor for an infectious disease work-up. Halley has been experiencing generalized pain and nausea for the past 3 weeks, and recently developed a fever. The doctors at the urgent care center, and then the emergency department, were perplexed by her presentation. You have been ordered to perform several tests: complete blood count (CBC) , C-reactive protein (CRP) , eosinophil sedimentation rate (ESR) , blood culture, urine culture, and a lumbar puncture with cerebral spinal fluid (CSF) culture and cell count. You are also collecting a sputum culture, as well as swabbing her nose with a culturette, and performing a nasal wash. -Halley's urine for culture was collected using a clean-catch method.After inoculation and incubation,several organisms were identified in amounts that were specified as normal microbial flora.What does this mean?


A) Halley is superinfected and needs immediate treatment.
B) It is impossible to interpret these results,and another clean-catch urine specimen needs to be cultured.
C) The results point to an infection,but due to multiple organisms,further testing needs to be done to isolate the offending microbe.
D) This is a common finding due to contamination of a clean-catch urine sample with normal microbiota from the urogenital area.

E) A) and B)
F) All of the above

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The Quellung test involves


A) a precipitation reaction.
B) an agglutination reaction.
C) a fluorescent reaction.
D) a lysis reaction.
E) a radioimmunoassay.

F) A) and D)
G) C) and D)

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Serological tests should have low sensitivity and specificity.

A) True
B) False

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When minute samples of DNA need to be genetically analyzed for identification purposes,which test can be effectively used?


A) direct fluorescence antibody
B) Widal
C) Weil-Felix reaction
D) PCR
E) direct antigen reaction

F) B) and E)
G) C) and D)

Correct Answer

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Please read the clinical scenario, and then answer the question that follow to become familiar with the traditional NCLEX question format. Tyler is a 16-year-old male who has come into your clinic with a low-grade fever, and large and painful red sores around his lips. He has a PCR test pending that was swabbed from the sores, as well as a blood culture with Gram stain, and nasal wash for examination by microscope. -Tyler's results came back as being positive for Herpes simplex virus.Tyler's mother is mortified,as she does not believe her son is sexually active.After you explain that the presence of HSV does not imply sexual contact,you offer that the results are accurate.Which of the following reasons best demonstrates this line of thinking?


A) Signs and symptoms don't lie.
B) PCR tests are highly sensitive and specific.
C) The ELISA is an excellent screen for HSV.
D) Apologize for being so certain,because further testing must be done.

E) A) and B)
F) A) and C)

Correct Answer

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