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Which of the following is not true about control of gene expression?


A) In bacteria it allows them to adopt to changing environments.
B) In multicellular organisms it is critical for development.
C) In bacteria it allows them to replicate without control.
D) In multicellular organisms it allows them to maintain homeostasis.
E) In multicellular organisms it allows them to function as a wholE.

F) D) and E)
G) B) and E)

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Transcriptional control-proteins increase the rate of transcription by binding to


A) mRNA sequences within the DNA.
B) tRNA sequences within the DNA.
C) operator sequences within the DNA.
D) promotor sequences within the DNA.
E) enhancer sequences within the DNA.

F) A) and C)
G) None of the above

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Your research project involves the characterization of a recently identified transcription factor.As part of your project,you want to determine if this transcription factor binds directly to any of the general transcription factors.Unfortunately,however,you are having trouble expressing and purifying the full length protein.Which of the following options may help you achieve your research goal?


A) Since transcription domains cannot be separated,you will need to abandon this experiment and try a vastly different approach to your question.
B) Express large amounts of the general transcription factors.
C) Since transcription domains can be separated,try to express and purify only the DNA-binding domain.
D) Since transcription domains can be separated,try to express and purify only the activation domain.

E) None of the above
F) A) and D)

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In __________ organisms,the primary function of a gene in a cell is to participate in regulating the body as a whole and not to responding to the cell's immediate environment.


A) multicellular
B) diploid
C) bacterial
D) prokaryotic

E) A) and C)
F) C) and D)

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In eukaryotes,the binding of TFIID is followed by the binding of a number of other general transcription factors.Which of the following lists the correct order of binding of these factors?


A) TFIIA,TFIIB,TFIIE,TFIIF,TFIIH
B) TFIIE,TFIIF,TFIIA,TFIIB,TFIIH
C) TFIIF,TFIIH,TFIIB,TFIIE,TFIIA
D) TFIIE,TFIIB,TFIIA,TFIIH,TFIIF

E) B) and D)
F) All of the above

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All regulatory proteins have common DNA binding motifs,which are particular bends in their protein chains that permit them to interlock with the


A) minor groove of the DNA helix.
B) major groove of the DNA helix.
C) outside groove of the DNA helix.
D) inside groove of the DNA helix.
E) hydrogen bonding groove of the DNA helix.

F) None of the above
G) A) and E)

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The TFIID complex is formed by


A) TATA-binding protein TBP and TAFs.
B) TATA-binding protein TBP,TAFs and RNA pol II.
C) TAFs and the core promoter.
D) TATA-binding protein and activators.

E) A) and B)
F) A) and C)

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Certain proteins can bind to specific DNA regulatory sequences by


A) entering the major groove of the DNA and reading the nucleotide base pairs.
B) entering the minor groove of the DNA and reading the nucleotide base pairs.
C) entering the major groove of RNA and reading the nucleotide base pairs.
D) entering DNA's major groove by using DNA polymerase and reading the nucleotide base pairs.
E) entering DNA's minor groove by using DNA polymerase and reading the nucleotide base pairs.

F) A) and C)
G) C) and D)

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A protein that initiates the transcription of genes which allow the use of non-glucose molecules.


A) operon
B) repressor
C) promoter
D) operator
E) CAP

F) D) and E)
G) A) and E)

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Regulatory proteins can identify specific sequences on the DNA double helix without unwinding the helix.This is accomplished by


A) inserting DNA promoters into either the major groove or the minor groove of the double helix where the edges of the nitrogen bases protrude.
B) inserting DNA-binding motifs into the minor groove of the double helix where the edges of the nitrogen bases protrude.
C) inserting DNA polymerase into the major groove of the double helix where the edges of the nitrogen bases protrude.
D) inserting RNA polymerase into the major groove of the double helix where the edges of the nitrogen bases protrude.
E) inserting DNA-binding motifs into the major groove of the double helix where the edges of the nitrogen bases protrudE.

F) A) and D)
G) A) and E)

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Which of the following is the hallmark of multicellular organisms?


A) grow and divide rapidly
B) cells adjust quickly to outside environment
C) homeostasis
D) quickly synthesize amount and type of enzymes according to available nutrients
E) respond by gene action to oxygen availability

F) A) and D)
G) A) and C)

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Enhancers are the binding sites for the


A) promotors of DNA synthesis.
B) suppressor factors.
C) co-activation factors.
D) mediator factors.
E) specific transcription factors.

F) A) and D)
G) C) and E)

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If you were able to look very closely at a portion of DNA and find methylated histones,you would


A) be mistaken since only DNA can be methylated,not histones.
B) be looking at a region of active chromatin.
C) be looking at a region of inactive chromatin.
D) be looking at a chromatin remodeling complex.

E) A) and B)
F) All of the above

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Proteins that bind to regulatory sequences have shapes that fit into the


A) promoter.
B) operator.
C) operon.
D) minor groove of DNA.
E) major groove of DNA.

F) A) and B)
G) A) and E)

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Elucidation of the histone code might


A) allow us to turn specific genes on or off.
B) allow the determination of nucleosome composition.
C) lead to chromatin remodeling.
D) allow us to control translation.

E) None of the above
F) All of the above

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Which of the following statements regarding primary transcripts in eukaryotes is correct?


A) The primary transcript is composed of RNA polymerase and associated histones.
B) The primary transcript has the exons removed and the introns retained for translation.
C) The primary transcript is a faithful copy of the entire gene including exons and introns.
D) The primary transcript is a faithful copy of the gene,but the introns have been removeD.
E) The primary transcript is a faithful copy,but the exons have been removed.

F) A) and C)
G) B) and E)

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The maintenance of a constant environment in a cell is called ___________.


A) active transport
B) homeostasis
C) gene expression
D) translation

E) B) and C)
F) B) and D)

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Although the specific mechanism of RNA interference has not been fully defined,it involves


A) double stranded RNA interference with mRNA.
B) double stranded RNA interference with DNA.
C) double stranded DNA interference with mRNA.
D) double stranded mRNA interference with DNA.

E) All of the above
F) A) and B)

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The main form of glucose repression of the lac operon is


A) induction.
B) repression.
C) inducer exclusion.
D) the CAP/cAMP system.

E) All of the above
F) A) and C)

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In gene regulation,negative control is exerted by a(n)


A) activator.
B) operon.
C) promoter.
D) regulator.
E) repressor.

F) A) and E)
G) B) and D)

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