Question 1

DNA restriction enzymes are labeled I and II.Type I restriction enzymes

Accepted Answer

A)  cut one strand at random points along the length of the DNA molecule.
B)  make simple cuts across both DNA strands.
C)  recognize palindromic sequences.
D)  cut at multiple cloning sites along the length of the DNA molecule.E) B) and D)
F) C) and D)

Question 2

A search for sequences that are complementary to the desired sequence of a DNA fragment uses a technique called

Accepted Answer

A)  plasmid insertion.
B)  vector extraction.
C)  cloning.
D)  electrophoresis.
E)  hybridization.F) None of the above
G) All of the above

Question 3

What of the following is a potential disadvantage of using transgenic plants for vaccine production?

Accepted Answer

A)  large-scale production costs
B)  inability to introduce new or multiple transgenes by sexual crossing of plants
C)  inability to produce the antibodies in edible plants such as potatoes
D)  risk of contamination with herbicides and pesticidesE) A) and C)
F) B) and C)

Question 4

A polymerase chain reaction (PCR) involves three steps.The correct order of those steps is

Accepted Answer

A)  denaturation,annealing of primers,and then primer extension.
B)  annealing of primers,denaturation,and then primer extension.
C)  primer extension,annealing of primers,and then denaturation.D) None of the above
E) A) and B)

Question 5

Autonomous auxiliary DNA circles found in bacteria;easily enter the organism they are found in;used as a vehicle to transport desired foreign genes.

Accepted Answer

A)  sticky ends
B)  RFLP
C)  PCR
D)  plasmids
E)  transgenic organismsF) A) and B)
G) C) and D)

Question 6

Two strands of DNA that have been cut by an endonuclease can be sealed together by a(n)

Accepted Answer

A)  polymerase enzyme.
B)  ligase enzyme.
C)  exonuclease enzyme.
D)  protease enzyme.
E)  methylase enzymE.F) B) and C)
G) A) and E)

Question 7

Enzymes that cleave DNA at specific sites are called ______________.

Accepted Answer

A)  vectors
B)  peptidases
C)  restriction endonucleases
D)  DNAsesE) C) and D)
F) B) and C)

Question 8

The development of PCR has revolutionized molecular biology and has led to numerous advances in basic research,as well as in medicine and forensic science.Which of the following areas does not rely on PCR?

Accepted Answer

A)  diagnosis of genetic diseases
B)  detection of infectious disease
C)  generation of hybrid plants
D)  evolutionary biologyE) C) and D)
F) None of the above

Question 9

Vectors are vehicles of transport for foreign DNA fragments into host cells,and are usually either a virus or a bacterial

Accepted Answer

A)  plasmid.
B)  protist.
C)  yeast.
D)  plant cell.
E)  human cell.F) A) and B)
G) A) and C)

Question 10

In addition to bacteria,_______ can also be used as vectors to insert foreign DNA into host cells and create recombinant genomes.

Accepted Answer

A)  viruses
B)  plasmids
C)  RNA
D)  prionsE) B) and D)
F) A) and B)

Question 11

The development of a subunit vaccine for herpes and hepatitis B has potential in the future.The proposed subunit vaccine would

Accepted Answer

A)  incorporate part of the mRNA of either the herpes or hepatitis B with the cowpox virus.
B)  incorporate part of the reverse transcriptases of either the herpes or hepatitis B with the cowpox virus.
C)  incorporate part of the DNA of either the herpes or hepatitis B with the cowpox virus.
D)  incorporate part of the protein-polysaccharide coat of either the herpes or hepatitis B with the cowpox virus.E) None of the above
F) B) and C)

Question 12

An enzyme that has been isolated from retroviruses is

Accepted Answer

A)  transverse ligase.
B)  reverse transcriptase.
C)  reverse endonuclease.
D)  recombinant RNAase.
E)  RNA polymerasE.F) B) and E)
G) A) and B)

Question 13

_________ is a human protein synthesized in small amounts that can dissolve blood clots.

Accepted Answer

A)  TPA
B)  fibrinogen
C)  DNA
D)  restriction endonucleaseE) All of the above
F) C) and D)

Question 14

Which of the following two enzymes are needed to construct a cDNA library?

Accepted Answer

A)  reverse transcriptase and DNA ligase
B)  reverse transcriptase and Taq polymerase
C)  DNA ligase and Taq polymerase
D)  Taq polymerase and RNA polymeraseE) A) and B)
F) B) and C)

Question 15

DNA fragments complementary to the DNA being investigated are referred to as

Accepted Answer

A)  rDNA.
B)  cDNA.
C)  mDNA.
D)  tDNA.E) A) and B)
F) A) and D)

Question 16

Golden rice was genetically engineered by Ingo Potrykus in Switerland by combining genes from other organisms into the genome of common white rice.Which organism from the list below was NOT used?

Accepted Answer

A)  daffodil
B)  wild rice
C)  beans
D)  Agrobacterium
E)  AspergillusF) C) and D)
G) All of the above

Question 17

Gel ________ is a process that separates DNA or protein fragments according to their size,by causing them to migrate within a gel in response to an electric field.

Accepted Answer

A)  duplication
B)  blotting
C)  electrophoresis
D)  migrationE) All of the above
F) A) and C)

Question 18

When compared with non-cancerous cells of the same tissue,cancer cells exhibit a number of changes in gene transcription.To identify genes that are differentially expressed in cancer cells versus non-cancer cells,one could perform

Accepted Answer

A)  subtractive hybridization.
B)  a northern blot.
C)  RFLP analysis.
D)  PCR.E) A) and D)
F) A) and B)

Question 19

Single-stranded ends generated by the same restriction enzyme are complementary to each other.They can be joined together,

Accepted Answer

A)  even when the source of the DNA is different.
B)  only when the source of the DNA is the same.
C)  but the "sticky ends" will most likely have to be modified.
D)  but the hybridization of the two ends may cause a problem with cloning.
E)  only if the subunits have been methylated.F) C) and D)
G) B) and E)

Question 20

Ends of DNA fragments produced by asymmetric cleavage with restriction endonucleases;same ends are produced by the same enzyme irrespective of the source of the DNA.

Accepted Answer

A)  sticky ends
B)  RFLP
C)  PCR
D)  plasmids
E)  transgenic organismsF) A) and B)
G) C) and E)

Exam 17: Biotechnology

Vectors are vehicles of transport for foreign DNA fragments into host cells,and are usually either a virus or a bacterial

Correct AnswerverifiedShow Answer

Which of the following two enzymes are needed to construct a cDNA library?

Correct AnswerverifiedShow Answer

Enzymes that cleave DNA at specific sites are called ______________.

Correct AnswerverifiedShow Answer

Ends of DNA fragments produced by asymmetric cleavage with restriction endonucleases;same ends are produced by the same enzyme irrespective of the source of the DNA.

Correct AnswerverifiedShow Answer

_________ is a human protein synthesized in small amounts that can dissolve blood clots.

Correct AnswerverifiedShow Answer

Gel ________ is a process that separates DNA or protein fragments according to their size,by causing them to migrate within a gel in response to an electric field.

Correct AnswerverifiedShow Answer

Golden rice was genetically engineered by Ingo Potrykus in Switerland by combining genes from other organisms into the genome of common white rice.Which organism from the list below was NOT used?

Correct AnswerverifiedShow Answer

In addition to bacteria,_______ can also be used as vectors to insert foreign DNA into host cells and create recombinant genomes.

Correct AnswerverifiedShow Answer

Autonomous auxiliary DNA circles found in bacteria;easily enter the organism they are found in;used as a vehicle to transport desired foreign genes.

Correct AnswerverifiedShow Answer

An enzyme that has been isolated from retroviruses is

Correct AnswerverifiedShow Answer

When compared with non-cancerous cells of the same tissue,cancer cells exhibit a number of changes in gene transcription.To identify genes that are differentially expressed in cancer cells versus non-cancer cells,one could perform

Correct AnswerverifiedShow Answer

A search for sequences that are complementary to the desired sequence of a DNA fragment uses a technique called

Correct AnswerverifiedShow Answer

The development of a subunit vaccine for herpes and hepatitis B has potential in the future.The proposed subunit vaccine would

Correct AnswerverifiedShow Answer

Two strands of DNA that have been cut by an endonuclease can be sealed together by a(n)

Correct AnswerverifiedShow Answer

Single-stranded ends generated by the same restriction enzyme are complementary to each other.They can be joined together,

Correct AnswerverifiedShow Answer

DNA fragments complementary to the DNA being investigated are referred to as

Correct AnswerverifiedShow Answer

What of the following is a potential disadvantage of using transgenic plants for vaccine production?

Correct AnswerverifiedShow Answer

DNA restriction enzymes are labeled I and II.Type I restriction enzymes

Correct AnswerverifiedShow Answer

The development of PCR has revolutionized molecular biology and has led to numerous advances in basic research,as well as in medicine and forensic science.Which of the following areas does not rely on PCR?

Correct AnswerverifiedShow Answer

A polymerase chain reaction (PCR) involves three steps.The correct order of those steps is

Correct AnswerverifiedShow Answer

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