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Vast numbers of microorganisms are being genetically characterized without culturing them in the laboratory today through ______.


A) column chromatography
B) metagenomic sequencing
C) mass spectrometry and proteomics
D) polymerase chain reaction

E) A) and D)
F) A) and C)

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A collection of organisms, together with the physical and chemical factors in their environment, are called a/an ______.


A) ecosystem
B) lithosphere
C) biosphere
D) atmosphere
E) hydrosphere

F) C) and D)
G) All of the above

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The mutualistic partnership between plant roots and microbes is ______.


A) rhizopods
B) mycorrhizae
C) basidia
D) hyphae
E) lichens

F) A) and B)
G) A) and C)

Correct Answer

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Which of the following types of microbes is most likely to be found in the sediment of Lake Michigan?


A) Anaerobes; oxygen is least abundant in the sediment layer
B) Autotrophs; their carbon source is dissolved carbon dioxide
C) Mesophiles; temperatures in the hypolimnion support growth in the 4-9°C range
D) Cyanobacteria; seasonal upwelling of nutrients can cause dramatic increases in their numbers

E) B) and D)
F) None of the above

Correct Answer

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Which of the following statements regarding red tides is incorrect?


A) Red tides result from an overgrowth of dinoflagellates.
B) Toxins produced by dinoflagellates are skin irritants.
C) Red tides are also known as harmful algal blooms (HABs) .
D) Dinoflagellates produce a toxin that causes paralytic shellfish poisoning.

E) A) and C)
F) C) and D)

Correct Answer

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Which of the following organisms anaerobically reduces sulfates to hydrogen sulfide or metal sulfide?


A) Nitrosococcus
B) Desulfovibrio
C) Thiobacillus thiooxidans
D) Rhizobium
E) Thiobacillus ferrooxidans

F) C) and D)
G) A) and E)

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Looking at the use of antibiotics as growth promoters in livestock through the lens of "One Health," which of the following statements best describes the interrelationship of these components of the biosphere?


A) Choosing a vegetarian lifestyle goes a long way to avoid risks associated with antibiotic- treated livestock.
B) Spontaneous antibiotic resistance that arises in animals can be rapidly spread to humans who work on factory farms.
C) The broad range of antibiotics available will provide widespread protection from pathogenic microbes.
D) Livestock can be more susceptible to infectious diseases because of the energy required for such rapid growth.

E) C) and D)
F) A) and C)

Correct Answer

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The majority of Earth's water is found in ______.


A) oceans
B) soil moisture
C) ground water
D) icecaps and glaciers
E) freshwater lakes

F) A) and B)
G) None of the above

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Nutrient deficient aquatic ecosystems are called ______.


A) autotrophic
B) oligotrophic
C) eutrophic
D) hypotrophic
E) epitrophic

F) C) and D)
G) B) and E)

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Which microorganism is considered phytoplankton?


A) Nitrogen ?xing bacteria
B) Sulfate reducers
C) Cyanobacteria
D) Mycorrhizae

E) None of the above
F) A) and B)

Correct Answer

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The combustion of fossil fuels, such as coal, oil, and gas, have contributed to the steady increase in atmospheric CO? gas over the last 25 years.

A) True
B) False

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Considering the increase in tick-borne diseases in the context of "One Health," which of the following statements is correct?


A) Lyme disease has spread from deer to humans upon increasing development of the forested environments usually inhabited by deer.
B) Ticks carrying multiple pathogens reproduce at a slower rate, rendering them less likely to infect humans.
C) Borrelia, Babesia, and Ehrlichia can rapidly adapt to changing insect vectors, thereby increasing their populations as they move from ticks to mosquitoes.
D) Ticks carrying Borrelia are likely to encounter hosts already infected with Babesia or Ehrlichia, resulting in ticks that co-infect with multiple pathogens.

E) A) and D)
F) A) and C)

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The zone of soil around roots of plants is called the ______.


A) ionosphere
B) rhizosphere
C) biosphere
D) hydrosphere
E) lithosphere

F) All of the above
G) A) and B)

Correct Answer

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The conversion of nitrate (NO3-) to nitrogen gas (N2) is called ______.


A) ammonification
B) nitrification
C) denitrification
D) nitrogen fixation
E) photosynthesis

F) C) and D)
G) D) and E)

Correct Answer

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You have been diagnosed with neurotoxic poisoning after having ingested an algal toxin, called brevetoxin, produced by Karenia brevis. In which food did you likely consume this?


A) Oysters
B) Tomatoes
C) Canned tuna
D) Freshwater trout

E) B) and D)
F) All of the above

Correct Answer

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Most of the organic sulfur is located in _______.


A) nucleotides and nucleic acids
B) monosaccharides and carbohydrates
C) fatty acids and lipids
D) amino acids and proteins

E) All of the above
F) A) and D)

Correct Answer

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You are preparing a garden and want to provide the best environment for your plants. Which of the following would you add to the potting soil?


A) Sulfur oxidizers
B) Mycorrhizae inoculant
C) Bacteriophage
D) Coliform bacteria

E) A) and B)
F) None of the above

Correct Answer

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Which of the following statements regarding biogeochemical cycles is incorrect?


A) Elements cycle through biotic and abiotic environments.
B) Recycling keeps nutrients in balance.
C) All elements originate from a nonliving, long-term reservoir.
D) All organisms directly participate in recycling.
E) Cycles are simple systems between living and nonliving components.

F) B) and E)
G) D) and E)

Correct Answer

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Plastic marine debris was collected from multiple sites in the ocean and examined with electron microscopy and next-generation sequencing. These techniques revealed microbial communities growing on the plastics. Which of the following statements is correct regarding these communities?


A) At least one member of the community must synthesize an enzyme to degrade the hydrocarbon comprising the plastic.
B) There are no bacteriophage that use these microbes as hosts, as viruses are rare in marine ecosystems.
C) The community within the plastic must be very similar to the community in the surrounding ocean waters.
D) These communities are very likely to be pure cultures, as plastics have been synthesized precisely because they are resistant to microbial degradation.

E) A) and C)
F) B) and C)

Correct Answer

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The study of the practical uses of microbes in various industries and technologies is ______.


A) industrial microbiology
B) bioengineering
C) microbial ecology
D) biotechnology
E) applied microbiology

F) B) and E)
G) A) and C)

Correct Answer

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